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KMID : 0525720150200030189
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Journal of Chitin and Chitosan
2015 Volume.20 No. 3 p.189 ~ p.195
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Physicochemical Properties and in vitro Dissolution of Alginic Acid Bead Containing α-Amylase
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Gu Yul-Ri
Park Hye-Mi
Hong Joo-Heon
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Abstract
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Abstract This study was conducted to examine the physicochemical properties and in vitro dissolution of the alginic acid bead containing 1% α-amylase. We prepared alginic acid bead according to the different concentration of CaCl2 (0.1, 0.3, and 0.5 M) and alginic acid (0.5, 1.0, and 2.0%). The lightness and yellowness were increased as CaCl2 concentration increase. However, the redness was no significant differences between CaCl2 and alginic acid concentration. The width and height length of alginic acid bead were increased as CaCl2 and alginic acid concentration increase. The peak stress and gel strength of alginic acid bead were increased as CaCl2 concentration increase. The α-amylase activity of 2% alginic acid and 0.3 M CaCl2 bead (363.49 unit/g) was higher than that of the others (287.04~362.61 unit/g). The in vitro dissolution was measured for five hours in pH 1.2 simulated gastric fluid, and pH 6.8 and pH 7.4 simulated intestinal fluids, using a dissolution tester at 37 with 50 rpm agitation. The α- amylase survival in the alginic acid bead was 104.76 unit/g (2% alginic acid and 0.1 M CaCl2) and it was very effectively controlled. There results suggest that the 2% alginic acid and 0.1 M CaCl2 bead may be a useful for functional food material in the food industry.
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KEYWORD
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Alginic acid bead' Amylase, in vitro dissolution, Physicochemical properties
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